The Arthanari Lab at Dana-Farber Cancer Institute focuses on protein-protein interactions (PPIs) as crucial therapeutic interventions. The lab employs structure-guided approaches to characterize and validate interactions within disease models, utilizing a combination of techniques such as NMR spectroscopy, NMR-based fragment high-throughput screening, and biophysical cell-based assays to map hotspots of interaction interfaces. The primary research areas include critical interactions involving transcription factors and the general transcriptional machinery, the Mediator complex, and translation initiation machinery, particularly in the context of dysregulation in cancer states. The lab aims to leverage NMR-derived metabolomics data for identifying novel metabolite disease markers and new potential therapeutic targets. Current projects also involve the development of innovative NMR methods for fragment screening and metabolite fingerprinting, including novel pulse sequences and labeling strategies, to expand the applicability of NMR techniques to larger systems.