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Achillefs Kapanidis is a professor in the Biological Physics group at Oxford Photonics, where his research focuses on understanding the mechanisms of molecular machines and gene expression using single-molecule biophysical methods and biochemistry. His primary experimental approach involves single-molecule fluorescence spectroscopy imaging, which is part of an expanding family of techniques that allow for the precise localization of single molecules to measure nanometre-scale distances and study molecular interactions in real time, both in vitro and in living cells. He is actively working on developing methods that incorporate novel instruments for biosensing assays. His specific interest lies in 'super-resolution' imaging methods that are revolutionizing microscopy by breaking the diffraction limit of optical microscopy. These advances provide high-resolution insights into the machines operating within living cells.